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aav9-cag-gfp  (Vector Biolabs)


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    Vector Biolabs aav9-cag-gfp
    Aav9 Cag Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aav9-cag-gfp/AAV9-CAG-GFP/custom%407076%4041390827
    Average 95 stars, based on 10 article reviews
    aav9-cag-gfp - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Over Expression:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Plasmid Preparation:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Expressing:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Bioprocessing:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Virus:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Recombinant:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    MTT Assay:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Mouse Assay:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Control:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Transfection:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Construct:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml

    Imaging:

    Article Title: Egr1 is a sex-dependent regulator of neuronal chromatin, structural plasticity, and behaviour.
    Article Snippet: the experimental overexpression AAV containing a smaller iteration of this enhancer (WPRE3) due to space limitations which retains a high degree of enhancer activity66. All viruses were purchased from Vector Biolabs (overexpression: AAV-258146, eGFP control: 7076; knockdown: shAAV-258146, scramble control: 7045) and were provided in PBS solution with 5% glycerol at a titre of ~1013 genome copies/ml



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    (A) Representative coronal sections in a 3-day-old marmoset show the widespread transduction pattern following a PC87 injection of <t>AAV9-CAG-GFP.</t> The side view of the extracted central nervous system highlights broad GFP labeling. Across all regions, the most abundant labeling is in the lower layers. (B–E) Detailed view of the insets shown in (A), highlighting the labeling pattern in the temporal, prefrontal, frontal, and parietal regions. Most labeled cells in the cortex are neurons, based on morphology and co-expression of NeuN (B, white arrowheads in yellow inset). Labeling in more superficial layers is almost entirely restricted to apical dendrites from lower-layer neurons (B, pink inset). The density of transduced neurons varied across cortical depths (dashed boxes in B–E). GFP+/NeuN+ cells were located in similar layers across regions, but the proportion of transduced neurons varied with depth, with up to 80% of cells being transduced at certain depths. (F–I) Cell labeling was observed in additional structures, such as the olfactory bulbs (OBs; A-1), striatum (Str; A-3, A-4, and F), hippocampus and choroid plexus (Hipp and CP, respectively; A-5 and G), and cerebellum (A-7 and I). Strong fiber labeling was observed in the thalamus (Th; A-5 and H) and white matter paths, such as corticofugal pathways within the internal capsule (F). Scale bars: (A) 1 mm, (B–H) 500 μm, and (I) and insets in (G) and (H), 250 μm. FIVI volume: 60 μL.
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    Image Search Results


    (A) Representative coronal sections in a 3-day-old marmoset show the widespread transduction pattern following a PC87 injection of AAV9-CAG-GFP. The side view of the extracted central nervous system highlights broad GFP labeling. Across all regions, the most abundant labeling is in the lower layers. (B–E) Detailed view of the insets shown in (A), highlighting the labeling pattern in the temporal, prefrontal, frontal, and parietal regions. Most labeled cells in the cortex are neurons, based on morphology and co-expression of NeuN (B, white arrowheads in yellow inset). Labeling in more superficial layers is almost entirely restricted to apical dendrites from lower-layer neurons (B, pink inset). The density of transduced neurons varied across cortical depths (dashed boxes in B–E). GFP+/NeuN+ cells were located in similar layers across regions, but the proportion of transduced neurons varied with depth, with up to 80% of cells being transduced at certain depths. (F–I) Cell labeling was observed in additional structures, such as the olfactory bulbs (OBs; A-1), striatum (Str; A-3, A-4, and F), hippocampus and choroid plexus (Hipp and CP, respectively; A-5 and G), and cerebellum (A-7 and I). Strong fiber labeling was observed in the thalamus (Th; A-5 and H) and white matter paths, such as corticofugal pathways within the internal capsule (F). Scale bars: (A) 1 mm, (B–H) 500 μm, and (I) and insets in (G) and (H), 250 μm. FIVI volume: 60 μL.

    Journal: Cell reports

    Article Title: Targeted gene transfer into developmentally defined cell populations of the primate brain

    doi: 10.1016/j.celrep.2025.116756

    Figure Lengend Snippet: (A) Representative coronal sections in a 3-day-old marmoset show the widespread transduction pattern following a PC87 injection of AAV9-CAG-GFP. The side view of the extracted central nervous system highlights broad GFP labeling. Across all regions, the most abundant labeling is in the lower layers. (B–E) Detailed view of the insets shown in (A), highlighting the labeling pattern in the temporal, prefrontal, frontal, and parietal regions. Most labeled cells in the cortex are neurons, based on morphology and co-expression of NeuN (B, white arrowheads in yellow inset). Labeling in more superficial layers is almost entirely restricted to apical dendrites from lower-layer neurons (B, pink inset). The density of transduced neurons varied across cortical depths (dashed boxes in B–E). GFP+/NeuN+ cells were located in similar layers across regions, but the proportion of transduced neurons varied with depth, with up to 80% of cells being transduced at certain depths. (F–I) Cell labeling was observed in additional structures, such as the olfactory bulbs (OBs; A-1), striatum (Str; A-3, A-4, and F), hippocampus and choroid plexus (Hipp and CP, respectively; A-5 and G), and cerebellum (A-7 and I). Strong fiber labeling was observed in the thalamus (Th; A-5 and H) and white matter paths, such as corticofugal pathways within the internal capsule (F). Scale bars: (A) 1 mm, (B–H) 500 μm, and (I) and insets in (G) and (H), 250 μm. FIVI volume: 60 μL.

    Article Snippet: AAV9-CAG-GFP , Addgene , 37825-AAV9.

    Techniques: Transduction, Injection, Labeling, Expressing, Olfactory